According to the difference of bacterial population category, biodegradation can be divided into two types: aerobic biodegradation and anoxic biodegradation. The result of biodegradation results in the following alteration of oil: the quantity of ①c1-c4 gaseous hydrocarbon decreases, until it is completely lost, the gas-oil ratio is significantly reduced, and the liquid hydrocarbons above ②C10 in accordance with the potential of anti-biodegradation, sequential degradation disappears; ③ crude oil density increases, viscosity thickens, sometimes sulfur content increases, oily deterioration, Become heavy viscous oil. In view of the different anti-biodegradation potential of hydrocarbons with different composition and molecular structure, the lower the degradation potential of hydrocarbons, the higher the degradation rate, the more sensitive to biodegradation, and vice versa. Thus, according to the sequence of degradation of different biomarkers, Peters (K.e.peters) and Moldovin (J.m.moldowan) presented a "quasi-phased" grading standard for measuring the degree of biodegradation of Petroleum in 1993: It should be noted that The sequence of the 3rd and 4th levels of the marked compounds belonging to the category of "strong biodegradation" may be substituted for different species of bacteria and/or different types of biodegradation processes.
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